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Experimental Study on the Anti-cancer Effect of Combined Usage of Sodium Cantharidinate and Camptothecin
Huang Danyi1, Wu Zhuanbin2, Huang Qin2, Zhang Mengjie1, Yang Hua1, Shi Jiahao1, Sheng Zhejin1, Fei Jian1*
1School of Life Science and Technology, Tongji University, Shanghai 200092, China;
2Shanghai Research Center for Model Organisms, Shanghai 201203, China
Abstract: Both sodium cantharidinate (SCA) and camptothecin (CPT) have been used for clinical tumor therapy. We studied the inhibition on tumor cell growth when combined SCA with CPT both in vitro and in vivo. The results showed that the combination of SCA and CPT enhanced the inhibitory effect of proliferation of A549 lung cancer cell line and Hep3B hepatoma cell line in vitro. And the CI values of SCA in conjunction with CPT of different concentrations were less than 1， indicating a synergistic effect. Using xenograft model， our results showed that the joint application of SCA and CPT could significantly inhibit the proliferation and diffusion of A549 cells in zebrafish embryo compared with single drug treatment. The combination treatment had no obvious impact on the normal development of zebrafish in the experimental dosage. We also observed that CPT inhibited the fin development of zebrafish embryos， as well as the inhibitory effect on the angiogenesis. The results provided useful experimental data for the combination therapy for related tumors using SCA and CPT in clinic.
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