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Prediction and Bioinformatics Analysis of miRNA Target Genes in Populus tomentosa under Fungus Stress
Liao Weihua, Chen Zhong, Ye Meixia, Ma Huandi, Gao Kai, Lei Bingqi, An Xinmin*
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Tree and Ornamental Plant Breeding and Biotechnology Laboratory, Beijing Forestry University, Beijing 100083, China
Abstract: There are few reports profiling the regulatory mechanisms of miRNA in poplar defense responses during fungus stress. It is very important to predict miRNA target genes accurately and rapidly for revealing the regulation effect of miRNA in gene expression during plant-fungus interactions. According to the conservative property of miRNAs， prediction of miRNA target genes was applied with a miRNA target analysis server psRNATarget using previously deposited miRNA sequences from Populus trichocarpa. A total of 772 target genes from 347 miRNAs were identified from Populus tomentosa under the infection to stem blister canker. Those genes encode proteins involved in disease-resistant categories， like plant hormone signal transduction， plantpathogen interaction， glutathione metabolism， etc. miR393 regulates auxin signal in response to a variety of external stimulus through the post-transcriptional regulation of target genes. 11 target genes of miR393 were identified， mainly involving in auxin mediated signaling pathway. Among these target genes， 6 genes were differentially expressed in response to fungus stress， including SD1-13， CYP83B1， TIR1， AFB2， AFB3 and PSBR. These genes are key candidates for studying the biological function of miR393.
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