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Study of Relationship between Morphology and Mature Ability，Chromatin Configuration， H3K27me3 in Immature Oocytes
Liu Yong1, Zhang Ling1, Jia Erteng1, Hua Xueyang1, Li Manman1, Zhang He1, Ding Biao1,Wu Fengrui1, Wang Huili2, Li Wenyong1*
1Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, Fuyang Teachers College,Fuyang 236037, China;
2Institute of Livestock Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Abstract: This research is engaged in studying the correlation of morphology index with mature ability， chromatin configuration and the epigenetic patterns of immature oocyte， providing the reference for oocyte selec tion in livestock breeding and medical assisted reproduction. Experimental materials are immature oocytes which are from the mice treated with different hormone (N， P， H group). Homemade perforated culture dish (HPCD) is used to track and test the maturation ability of immature oocytes. Chromatin configuration and H3K27me3 level were detected with Hoechst33342 and antibody， respectively. The results showed that the oocyte with smaller cytoplasm in diameter was more suitable for maturation in the HPCD system [(71.63±1.02) μm (A form) vs (68.33±0.97) μm (B form)， P<0.05] in the group H. Nucleolus’ diameter and the thickness of the zona pellucida have nothing to do with maturation ability. Non-surround nucleolus (NSN) configuration ratio was higher than that of surround nucleolus (SN) (78.57% vs 21.43%) in A form oocytes， and SN configuration ratio was higher than that of NSN (65.12% vs 34.88%) in B form oocytes. H3K27 methylation had no significant differences in oocytes which were from N， P and H groups， but H3K27 methylation of immature oocyte in A form was significantly lower than B form in group N (P<0.05). There were no significant differences of H3K27 methylation between A form and B form in group P and group H. H3K27 methylation level of A form was significantly lower than that of B form (P<0.05) despite hormone treatment. In conclusion， the oocyte morphology could reflect its matural ability， chromatin configuration and H3K27 methylation of oocytes while levels of hormones affected the H3K27me3 level.
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