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In Vitro Effect of Acrylamide on Human Spermatozoa Functions
Zou Qianxing1, Chen Wen2, Weng Shiqi1, Luo Tao1*
1Institute of Life Science, Nanchang University, Nanchang 330031, China;
2Institute for Advanced Study, Nanchang University, Nanchang 330031, China
Abstract: The aim of this study is to explore the in vitro effect of acrylamide on the function of human spermatozoa. Different concentrations (0， 2， 10， 50， 250， 1 000 μmol/L) of acrylamide were exposed to human ejaculated spermatozoa in vitro. The viability， motility， capacitation， acrosome reaction (AR) and intracellular calcium concentration ([Ca2+]i) of acrylamide-treated spermatozoa were assessed by eosin-nigrosin staining， computer-aided sperm analysis， chlortetracycline staining and single sperm calcium imaging. The results indicated that all the concentrations of acrylamide used in this study had no significant effect on the viability， motility， capacitation， AR and [Ca2+]i of human spermatozoa. These results implied that in vitro exposure to acrylamide did not inhibit the function of human spermatozoa in a short time.
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