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Fluorescence of Green Fluorescent Protein was Disappeared in Cells Fixed with Methanol
Rong-Fang Li1,2, Hui Yuan1, Ding-Gan Liu2
1College of Animal Medical, Hunan Agricultural University, Changsha 410128,China; 2State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of
Abstract: It was found that the fluorescence of GFP in cells transfected with green fluorescent protein (GFP) gene disappeared when those cells were fixed with methanol， whereas those fixed with paraformaldehyde remained fluorescent brightly. Therefore it is suggested that methanol be avoided in fixing cells expressing GFP for immunohistochemistry.
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