Influencing Factors of the Semi-dry Electrophoretic Transfer and the Optimize Conditions

Abstract: To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot， the protein marker and purified recombination protein were used to analyzed. In the different electrophoretic transfer condition， the phenomenon of protein transmembrance was detected on the PVDF membrane. When the electric current is permanent， the transfer time and loading protein were the main causes in the excess transfer. It is demonstrated that the loading protein and transfer time in the transfer depend on the molecular mass and expressive abundance of protein which is detected.

CSTR: 32200.14.cjcb.2007.02.0026