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Establishment of Cell Model with Latent Adenovirus Infection
Juan Chen1, Bing Li2, Qi-Cai Liu2, Li-Hong Xiong3, Pi-Xin Ran1*
1Guangzhou Institute of Respiratory Disease, Frist Hosptial Affiliated Guangzhou Medical College, Guangzhou 510182, China; 2The Experimental Medical Research Center, Guangzhou Medical College, Guangzhou 510182, China; 3Dep
Abstract: The influences of latent adenovirus infection on the rat alveolar epithelial cells， and the relationship between latent adenovirus infection and airway disorder have been well documented. For illustrating the roles of adenovirus infection in the process of airway disorder， we constructed a eukaryotic expression vector for expressing adenovirus E1A protein and transfected into CCL149 cell. Cells stably expressing E1A protein were selected by G418 resistance. 109 G418-resistant clones were obtained， those clones were identified by PCR， RT-PCR， Western blot and immunocytochemistry. Among them four E1A-positive clones and nine control clones were verified as stable transfected clones. Primary study showed that E1A gene could inhibit the proliferation of CCL149 and reduce the cell of S phase and prolong the cells in the G1 phase at the same time E1A sensitizes cell to TNFα induced apoptosis of CCL149 cell.
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