Expression and Genetic Instability of KAI1 Gene in Chinese with Gastric Cancer

Abstract: The aim of the study was to study the expression of KAI1 protein and the genetic instability of KAI1 gene， and their relationship with clinical pathological behaviors in Chinese with gastric cancer， which will illustrate the evaluation of tumor metastasis and the prognosis in gastric carcinoma， and provide experimental basis for the mechanism of KAI1 gene. Techniques such as Envision immunohistochemistry and Leica-Qwin computer imaging techniques were used to assess the expression of KAI1 protein. DNA extraction from formalin-fixed paraffin-embedded tissues， polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)， ordinary silver stain were used to study microsatellite instability (MSI) and loss of heterozygosity (LOH) of locus D11S1344 and D11S1326. In our experiment， the expression rate of KAI1 protein was 55.4% (31/56). With the infiltration of tumor， the frequency of KAI1 protein appeared decrease tendency (P<0.01). Moreover， the frequency in lymph node metastasis cases was (83.9%) significantly higher than those without (20.0%， P<0.01). Stage TNM I+II (82.8%) also exhibited higher frequency of KAI1 protein than stage TNM III+IV (25.9%， P<0.01). However， none of 56 gastric cancers showed LOH or MSI at locus D11S1344 and D11S1326. The results indicated that the expression of KAI1 protein has great relationship with the infiltration of tumor， the lymph node metastasis and the prognosis of gastric carcinoma. There is rare LOH or MSI in KAI1 gene in gastric carcinoma.
    

CSTR: 32200.14.cjcb.2007.02.0016