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Detection of lin-4 mRNA in Caenorhabditis elegans Wild Type and Mutants via in Situ Hybridization
Ya-Jun Gao, Yu-Rong Yang*
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen 361005, China
Abstract: lin-4 is an important heterochronic gene as well as a microRNA which plays an crucial role in regulating the larval stage transition in Caenorhabditis elegans (C.elegans). To determine the function of lin-4 in the temporal and spatial development of C.elegans， we analyzed the expression of lin-4 mRNA in wild type and mutants of lin-14 and lin-28 via in situ hybridization. The results showed that lin-4 mRNA presented in the early embryos of wild type， and could not detected in the later embryos or L1 larvae. The level of it continued to increase through L2 to adult worms. During subsequent stages of development， it presented in the epidermis， and also in the germiline of adult worms. In lin-14 and lin-28 mutants， the expression of lin-4 mRNA pattern was same as wild type which implied lin-14 and lin-28 was the down streamed gene of lin-4.
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