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The Immunotherapeutic Antitumor Effect of Dendritic Cells Co-cultured with Cytokine Induced Killer Cells
Jie Zhuang, Hui Cao, Xiang-Lin Liu1, Shang-Quan Zhang2*
General Surgical Department, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China; 1Shanghai Zhong Ke Biotech Co., Shanghai 201318, China; 2Institute of Biochemistry and Cell Biology, Shanghai Ins
Abstract: To investigate the antitumor effects of DCCIK which is from dendritic cells were co-cultured with the cytokine induced killer cells (CIK). MTT assays were used to compare the cytotoxicity between DCCIK and CIK cells in vitro. DCCIK cells showed better antitumor cytotoxic effects than the CIK cells. The anticancer effects of DCCIK combined with chemotherapy or chemotherapy alone which were evaluated by using of these cells to treat the Balb/c nude mice which were implanted the A549 lung cancer， BEL-7404 liver cancer and A375 melenoma cells. Measurement of tumor size after 20 and 35 days of treatment， the result indicated that immunotherapy of DCCIK cells plus chemotherapy had a better antitumor effect than the chemotherapy alone. And it also suggested the DCCIK cells has a potential clinic value.
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