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The Display Technologies of Molecular Libraries
Yuan Zou, Jin-Biao Zhan*
Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, China
Abstract: Display technologies are the newly-developed methodology for functional genomics study. Display systems are used for the selection of coding elements (DNA or RNA) from libraries in which the individual peptide or protein as phenotype is physically associated with their genetic materials. Several methods have been devised and validated， both cell based， such as phage display and cell surface display， and cell-free， such as ribosome display， and mRNA display. The success of display selection relies on the ability to retrieve the genetic information along with the functional proteins. They are widely used in various ranges of applications， including: screening and identifying protein-ligand interactions， live vaccine development， epitope mapping， antibody engineering， biosensors development for diagnostic， industrial and environmental purposes and so on. This review briefly describes the principle of each display technology， and their applications.
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