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Comparison of Two Methods for the Nucleus Maturity Test in Mouse Oocytes
Jian-Ying Wang, Fa-Chun Wan, En-Liang Song, Xiu-Mei Zhang*
Key Lab of Animal Disease Control and Breeding, Animal Husbandry/Vet Institute,Shandong Academy of Agricultural Science, Jinan 250100, China
Abstract: To select a simple， fast and sensitive method for the nucleus maturity detection in mammalian oocytes， GV-stage oocytes were cultured in M2 culture medium， at 0 h， 4 h， 8 h and 12 h of culture， oocytes were collected and used for nucleus maturity test by SYTO staining and immunofluorescence technique respectively. The results were compared. We found that both SYTO dyeing and immunofluorescence technique can be used for the nucleus maturity detection in mouse oocytes， and there is none distinct difference， but SYTO dyeing method is faster， cheaper and more simple for nucleus maturity detection， and it also suits to vital staining and large-scale examination， thus SYTO dyeing can be used for the nucleus maturity detection in mammalian oocytes.
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