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Neuroprotective Effect of Phase II Enzyme Inducer CPDT on the Selective Motor Neuron Injury and Possible Mechanisms
Zhe Li, Xiao-Yun Liu1,2, Hui Bu 1,2, Bin Li 1,2, Yan-Su Guo1,2, Chun-Yan Li1,2*
1Department of Epilepsy, Hebei General Hospital, Shijiazhuang 050051, China; 1The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China; 2Hebei Province Institute of Cardiocerebrovascular Disease, Shijiaz
Abstract: On the basis of the model of selective motor neuron injury which induced by inhibitor of glutamate transporter， to investigate the neuroprotection effect and mechanism of phaseⅡenzyme inducer 5，6-dihydrocyclopenta[C]-1， 2-dithiole-3-thione (CPDT). Organotypic spinal cord slices of rat pup were divided into normal control group， THA model group (THA 100 μmol/L) and phaseⅡenzyme inducer CPDT (15， 30 μmol/L) treatment groups. Ventral α motor neurons?survival of spinal cord slices in each group was evaluated by immunohistochemical staining. The expression of NQO1 and ferritin in each group was evaluated by the method of RT-PCR， Western blot and assay of enzyme activity et al.. Compared with THA model group， the number of a motor neurons significantly increased with intervention of CPDT (15 μmol/L or 30 μmol/L). And the expression of antioxidant enzyme of NQO1 and heavy chain of ferritin increased significantly in CPDT treatment group. The result provided fundamental conditions for intervention on animal model of amyotrophic lateral sclerosis (ALS) and ALS patients.
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