Cisplatin on the Effect of Aquaporin 5 Protein Expression of SKOV3 and Its Regulation

Abstract: To investigate cisplatin on effect of aquaporin 5 (AQP5) expression of SKOV3 and regulation of PDTC that is NF-κB inhibitor by MTT， Western blot and RT-PCR. Following the concentration of cisplatin increasing， the expression of AQP5 protein (P=0.001) as well as NF-κB p65 in cytoplasm and karyon and IκBα in karyon， P=0.000， 0.000， 0.000， in SKOV3 incubated with cisplatin 24 h decrease. After SKOV3 incubated with 10 μg/ml cisplatin 6 h to 12 h， the protein expression of AQP5， NF-kB p65 in cytoplasm and karyon and IκBα in cytoplasm increase rapidly， but decrease suddenly at 24 h， remain the lower level until 72 h (P=0.000， 0.000， 0.000， 0.000). The more concentration and longer incubated time of PDTC， the more decreasing of AQP5 and mRNA (P=0.000， 0.000). There is a negative correlation between AQP5 protein and the inhibiting rate of SKOV3 induced by cisplatin (r=-0.598， 0.009)， and a negative correlation between the inhibiting rate induced by PDTC and AQP5 (r=-0.983， P=0.000) as well as AQP5 mRNA (r=-0.905， P=0.000)， and a positive correlation between AQP5 expression and NF-κB p65 as well as IκBα (r=0.894， 0.857; P=0.000， 0.000). These results suggest that AQP5 is related to SKOV3 cells proliferation and cisplatin may reduce AQP5 expression， which may involved in NF-κB gene regulation. AQP5 will be potential target for chemotherapy of ovarian carcinoma.
    

CSTR: 32200.14.cjcb.2008.06.0018