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Effects of High Magnetic Gravitational Environment on Expression and Distribution of Dynamin-2 in MG-63
Sheng-Meng Di, Ai-Rong Qian, Zong-Cheng Tian, Wei Zhang, Li-Fang Hu, Da-Chuan Yin, Hui-Yun Xu, Yuan-Yuan Weng, Peng Shang*
Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Faculty of Life Sciences, Northwestern Polytechnical University, Xi抋n 710072, China
Abstract: The aim of the research is to investigate the effects of high magnetic gravitational environment (HMGE) produced by a superconducting magnet on gene expression and distribution of dynamin-2 in human osteosarcoma cell line MG-63 cells. A special designed large gradient superconducting magnet which can produce three HMGE， namely 0 g (12 T)， 1 g (16 T) and 2 g (12 T) . After the cells were cultured under 0 g (12 T)， 1 g (16 T)， 2 g (12 T) and control environments for 24 h， Real Time PCR， Western blot and laser scanning confocal microscopy (LSCM) were utilized to detect the expression and distribution of dynamin-2. And the results showed that， compared to control group， dynamin-2 expression at mRNA level in MG-63 cells exposed to 0 g (12 T)， 1 g (16 T) and 2 g (12 T) conditions was increased by 6.43， 15.57 and 1.29 folds， respectively. However， at protein level， dynamin-2 expression in MG-63 cells exposed to 0 g (12 T) and 1 g (16 T) conditions was increased by 89% and 7% while that of 2 g (12 T) condition was decreased by 41% compared to control group. In addition， dynamin-2 was mainly diffused in the cytoplasm in MG-63 cells of control groups， so was that of 1 g and 2 g groups. But in 0 g group， the distribution of dynamin-2 exhibited trends towards accumulation in the perinuclear region. Taken together， the results suggest that HMGE influences expression and distribution of dynamin-2 in MG-63 cells.
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