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The Effects of the Combination of Ginsenoside Rg1， Cinnamic Acid and TanshinoneII A on the Proliferation and Genes Expression of MG-63 Cell
Song-Lin Shi, Guo-Hong Wang, Qi-Fu Li*, Zong-Yuan Ma, Chang-Jian Lin1, Zhen-Li Zhao
Key Laboratory of Ministry of Education for Cell Biology & Tumor cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China; 1State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College
Abstract: To explore the anticancer mechanisms and biological effects of the effective component of chinese herbs， the human osteosarcoma MG-63 cells were treated with the combination of 33 μg/ml ginsenoside Rg1， 296.32 μg/ml cinnamic acid and 0.3 μg/ml tanshinoneIIA (RCT) and the MG-63 cells treated with hexamethylene bisacetamide (HMBA) were investigated as positive control of induced differentiation. They were subjected to flow cytometry， immuncocytochemical assay and light microscopy. The analysis of cell growth curve and cell cycle revealed that RCT can markedly inhibit cell proliferation and arrest cells in G0/G1 phase. The inhibitory rate of cell growth was 72.37% after 7 days treatment by RCT. The results of immunocytochemical staining indicated that the expression of oncogenes including c-fos and c-myc were down-regulated， whereas the expression of antitumor genes including p27 and Rb were up-regulated. Similar results were obtained from HMBA treated MG-63 cells.
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