Acetylbritannilactone Inhibits Inflammatory Stimulus-mediated Adhesion between Endothelial Cells and Macrophages

Abstract: To investigate the effects of 1，6-O₂-diacetylbritannilactone (ABLO₂) on the activation of endothelial cell ECV304 and its interaction with macrophages treated with lipopolysaccharide (LPS)/interferon- (IFN-γ). Western blot analysis was adopted to measure the nuclear translocation of nuclear factor-κB (NF-κB) and the expression of some adhesion molecules. Electrophoretic mobility shift assay (EMSA) was used to detect DNA-binding activity of NF-κB in ECV304 cells pretreated with ABLO₂. The results showed that ABLO₂ inhibited the NF-κB activation by blocking IkB kinase (IKK) activation， and suppressing inhibitor of NF-κB (IκB) phosphorylation and degradation. In addition， ABLO₂ could inhibit the adhesion between endothelial cells and macrophages by decreasing the expression of vascular cell adhesion molecule-1 (VCAM-1)， osteopontin (OPN)， matrix metalloproteinase-9 (MMP-9) and tenascin-c. These results suggest that ABLO₂ is a potent agent to prevent vascular inflammatory disease in the vessel.

CSTR: 32200.14.cjcb.2008.06.0015