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Reprogramming Somatic Cells into Stem Cells Using Cell Extract
Hui Liu, Jiang Li, Xin-Yuan Liu, Qi-Jun Qian*
Xin Yuan Institute of Medicine and Biotechnology, College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
Abstract: Extensive investigation demonstrated that terminally differentiated somatic cells can be reversely reprogrammed into a pluripotent state， which obviates ethical concerns and legal restriction in embryonic stem (ES) cell research and ignites reborn enthusiasm on reprogramming for patient-specific pluripotent cells and regenerative medicine. This paper primarily elucidates reprogramming principle mediated by cellular extracts and its applicative prospect as well as cutting edge approaches of somatic cell reprogramming， including nuclear transfer into oocyte， cellular fusion between somatic cells and ES cells or embryonic carcinoma (EC) cells， ectopic expression of transcription factors and coculture of somatic cells with extracts from oocytes， ES cells or multipotent cancer cells.
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