The Inhibitory Effect of WDR79 RNAi on the Proliferation of Human Lung Cancer A549 Cells

Abstract: WDR79 is a telomerase holoenzyme which is notably enriched in Cajal bodies (CBs)， it facilitates the recruitment of telomerase to CBs in HeLa cells. We achieved down-regulation of WDR79 expression in A549 cells by conducting transient WDR79 siRNA transfection. Real-time PCR and Western blot assays were used to evaluate the mRNA and protein level of WDR79 gene. The telomerase/telomere colocalization was detected by immunofluorescence double labeling confocal laser scanning microscopy. The telomere length was detected by Southern blot. The proliferation of cells was analyzed by MTT assay. The cell cycle analysis and apoptosis were detected by flow cytometry (FCM). The results demonstrated that compared with the control siRNA and untreated groups， the mRNA and protein level of WDR79 were significantly decreased (P<0.05); The telomerase/telomere colocalization was abolished (P<0.05); The proliferation ability was inhibited significantly (P<0.05); WDR79 depletion caused decreased S phase cell and led to G1 phase cell cycle arrest (P<0.05). The biological effect of A549 cells may significantly inhibited by down-regulation of WDR79， indicating that WDR79 may serve as a potential candidate for the development of therapeutic target for lung adenocarcinoma.

CSTR: 32200.14.cjcb.2014.10.0009