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Isolated Culture， Identification and Differentiation Potential of Menstrual Blood-derived Mesenchymal Stem Cells
Yan Yan1, Dong Jianfeng1, Sang Yunxia1, Liu Zhaoqing2, Qi Nianmin1*
1Pharmacy College, Shanghai Jiao Tong University, Shanghai 200240, China;2Shanghai Kun’ai Biological Technology Co., LTD, Shanghai 201203, China
Abstract: Stem cell therapy can help cure many diseases. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In this research， we investigated the basic biological characteristics and differentiation potential of novel menstrual blood-derived mesenchymal stem cells (MenSCs). MenSCs specific markers were analyzed by a flow cytometer. Nestin expression was evaluated with immunofluorescence. The capacity of osteoblast and adipocyte to differentiate was evaluated. Our results showed that MenSCs were successfully isolated from female menstrual blood. The doubling rate of MenSCs was 32.2 h. MenSCs were positive for CD29， CD90， CD105， while negative for CD14， CD45， HLA-DR. Immunofluorescence showed that MenSCs exhibited nestin-positive characteristics. MenSCs were positive for Oil Red O staining after inducing by adipogenic differentiation medium. After exposure in osteogenetic differentiation media， collagen expression of MenSCs was higher at the early age and Alizarin Red staining evidenced that MenSCs exhibited osteocyte phenotypes after 2 weeks. In addition， ALP activity analysis illustrated that MenSCs was on the rise for 3 weeks. These results indicate that MenSCs have properties of low immunogenicity， high proliferation potential and multi-linage differentiation capacity. Our study suggests that MenSCs might be an ideal resource for stem cell therapy and regenerative medicine applications.
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