Electrotaxis of Mouse Epithelial Melanoma Cells on Three Different Matrixes

Xu Linfeng¹, Zhao Min^1,2, Wang Xiaoyan¹, Zhao Sanjun^1*

¹Regeneration Lab, Yunnan Normal University, Kunming 650500, China; ²Department of Dermatology and Department of Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, California 98517, USA

Abstract: Directed cell migration is a crucial behavior in development， regeneration and wound healing.The small directed electric fields (EF) were proved to be one of the important direction signals to giuid the cell migration. Furthermore， the physical and chemical properties of matrix would influence many aspects of the cell migration. In this study， using melanoma cell (B16-F10) as a model， we studied the electric fields guided cell migration on petri-dish， Matrigel and FNC. The results indicated that cells migrated toward cathode on each matrix，While the cells exhibited obviously different migration speed and persistence on different matrixes.

CSTR: 32200.14.cjcb.2014.06.0014