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Protective Effect and the Mechanism of L-theanine Against H2O2 Induced Injury in L02 Cells
Li Guilan1, Kang Jingjing2, Yin Zhimin2*
1College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China; 2Jiangsu Province Key Laboratory for Molecular and Medicine Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China
Abstract: The object of this study was to explore the protective effect and the mechanism of L-theanine against hepatocyte injury. The human hepatic L02 cells injured by H2O2 were used as a cell model. The protective effect of L-theanine against H2O2-induced hepatocyte injury was evaluated by MTT determination for cell viability， LDH assay， using flow cytometry for apoptosis rate and Western blot for protein expression of Caspase-3， PARP and the ratio of Bax/Bcl-2. The results showed that L-theanine attenuated the cell viability loss and LDH release induced by H2O2， and prevented L02 cells from H2O2-induced cell apoptosis. In addition， L-theanine mediated the anti-apotosis role by decreasing Caspase-3 activation， PARP cleave and Bax/Bcl-2 ratio. In conclusion， L-theanine helps to protect and cure the hepatocyte injury.
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