TRPV2 Activation Enhances the Expression of Nerve Growth Factor in Primary Cultured Astrocytes Under Oxygen-glucose Deprivation/Reoxygenation

Abstract: Nerve growth factor (NGF) is essential for neuronal growth and survival. We had addressed the effect of 2APB， an activator of TRPV2， on the NGF expression in astrocytes exposed to oxygen-glucose deprivation/reoxygenation. Primary cultured cortical astrocytes stimulated with or without 2APB (0.5 mmol/L) were treated with glucose-deprivation media under oxygen-glucose deprivation for 2 h and then incubated under normal culture condition for 48 h. The levels of NGF protein in astrocytes were analyzed by Western blot and NGF protein levels in astrocyte-conditioned media were measured by enzyme-linked immunosorbent assay (ELISA). 2APB increased the expressions of NGF protein in astrocytes under normal and reoxygenation condition. After 48 h reoxygenation， 0.5 mmol/L of 2APB enhanced the levels of NGF protein in astrocyte-conditioned media by 1.88-fold of the control. An inhibitor of c-Jun N-terminal kinase (JNK) suppressed the effect of 2APB on the NGF expression. We concluded that 2APB enhanced， via the JNK pathway， NGF expression in astrocytes. TRPV2 activation might have therapeutic potential for brain injury in ischemia/reperfusion.

CSTR: 32200.14.cjcb.2014.06.0010