Home > Browse Issues > Vol.36 No.6
Cloning and Expression Analysis of Anthocyanidin Synthase in Safflower
Liu Xiuming1,2, Yang Wenting1,2, Zhao Lidan1,2, Dong Yuanyuan1, Yao Na1, Wang Nan1, Guan Lili1, Li Haiyan1,2*, Li Xiaokun1,2*
1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University,Changchun 130118, China; 2College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
Abstract: In this study， the full-length cDNA sequence of anthocyanidin synthase (ANS) gene was cloned from flowers of Carthamus tinctorius L. (safflower) by RT-PCR and RACE techniques according to the sequences of transcriptome in safflower. The full-length cDNA of the ANS was 1 226 bp and included a whole open reading frame of 1 050 bp， encoding a polypeptide of 349 amino acids. The putative protein of the gene showed predicted molecular weight of 82.27 kDa with a theoretical pI of 5.09， and this gene contains typical AATAA tail signal sequence and Poly(A). The conserved structural domain analysis showed that it had the typical functional domains of ANS protein， containing 2-oxoglutarate and iron ion combination sites. Safflower ANS had high homology with other species according to the blasting and phylogenetic analysis， which indicated that safflower ANS was more related to ANS from Paeonia lactiflora Pall. Real-time PCR results indicated that relative expression of ANS gene was highest in early flowering period and blooming period.
CN
EN