Home > Browse Issues > Vol.36 No.6
Establishing Spermatogonial Stem Cell Lines of Mice at Different Ages
Zhou Hai1,2, Wu Chunlian1*, Li Jinsong2*
1School of Life Science, China West Normal University, Nanchong 637002, China; 2State Key Laboratory of Cell Biology,Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200031, China)
Abstract: Spermatogonial stem cells (SSCs) are a pool of cells which are capable of self-renewal and differentiation in male animals’ whole-life. We established several SSC lines of mice at different ages using three methods. The results showed that two methods (difference adherence and direct adherence) could be used to establish SSCs in the 6~7 d little mice， but the SSCs from 5~6 weeks’ mice were established only by the difference adherence. However， we did not get the SSCs lines of 31 weeks mice using the methods which were mentioned above other than culture on feeder cells. Finally， the functions of those SSCs were determined through analysis of spermatogonial stem cell lines methylation， specific genes expression and in vivo testis transplantation validation.
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