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Establishment and Analysis of Lung Cancer Cells H1299 with Stable B-Myb Over-expression
Cai Wei1,2, Wang Yitao1,2, Zhu Yuanyuan1,2, Wang Sen1,2, Zhu Huifang1,2, Lei Yunlong1,2, Li Yi1,2, Zhang Ying1,2, Zhang Chundong1,2*, Bu Youquan1,2*
1Department of Biochemistry and Molecular Biology, ChongQing Medical University, Chongqing 400016, China; 2Molecular Medicine and Cancer Research Center, ChongQing Medical University, Chongqing 400016, China
Abstract: B-Myb is one of members of the Myb family， and it plays critical roles in both cell cycle and cancer development. However， the role and molecular mechanism of B-Myb in lung cancer were still unclear. To investigate the role of B-Myb in lung cancer， H1299 cells with stable B-Myb over-expression were established. FACS and MTT results showed that stable over-expression of B-Myb led to decreased percentage of G1 phase cells， increased percentage of S phase cells and subsequently promoted cell proliferation. Colony formation assay and Transwell assay indicated that stable over-expression of B-Myb significantly enhanced the colony formation， invasion and migration abilities of H1299 cells. Quantitative RT-PCR results also demonstrated that B-Myb over-expression significantly up-regulated the expression of cell cycle related gene CCNA1. There were no significant changes in the expression of cell motility related downstream genes such as CD97 and MTSS1. Taken together， we have successfully established the B-Myb stable over-expressed cells and found that over-expression of B-Myb can promote proliferation， invasion， migration and colony formation abilities of lung cancer cells.
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