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In Vitro Study of Gadopentetate Acid Dimeglumine Labeled Human Umbilical Cord Mesenchymal Stem Cells Mediated by Three Different Transfection Agents Tracking under MR
Shuai Hanlin1, Lin Xiaowen1, Shi Changzheng2, Song Hong1, Luo Xin1*, Zhu Xiaohua1
1Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan University, Guangzhou 510630; 2 Medical Image Center, the First Affiliated Hospital of Jinan University, Guangzhou 510630
Abstract: Human umbilical cord tissue contains plenty of mesenchymal stem cells， which is the ideal stem cell seed source. How to isolate mesenchymal stem cells from umbilical cord and trace the biological behavior of stem cells by image techniques are current research focuses. The hUCMSCs from full-term pregnant women’s umbilical cord tissues were separated， purified and identified in this research. Furthermore， Gd-DTPA was transfected into mesenchymal stem cells through calcium phosphate， Effectene and Lipofectamine 2000. The change of labeled cell’s signal strength was detected by MR and the gadolinium ion concentration intra celluar were determined by inductively coupled plasma atomic emission spectrometry for evaluation of the transfection capacity of three reagents. In conclusion， the method of Effectene mediated Gd-DTPA marker hUCMSCs is best and this study provides the experimental foundation for Gd-DTPA labeled stem cells in vitro MR.
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