Home > Browse Issues > Vol.36 No.5
Isolation， Proliferation， Identification and Reprogramming of Mesenchymal Stem Cells Derived from Fetal Lung
Chi Ying#, Yang Shaoguang#, Xu Fangyun, Wang Youwei, Han Zhibo, Zhao Qinjun, Ma Fengxia,Chen Fang, Lu Shihong, Han Zhongchao*
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
Abstract: The research is about to reprogramme mesenchymal stem cells derived from fetal lung (FLMSCs) into induced pluripotent stem cells (iPS cells). At first， we used trypsin to digest the tissue block derived from fetal lung， and then we obtained fibroblast-like cells through the conventional culture method. Detected the immunophenotype and differentiation potential of fibroblast-like cells to confirm that they were FL-MSCs. The expression level of keratin was demonstrated by confocal immunoflurescence， and it was negative. The expression levels of c-Myc， Oct4， Nanog and Nestin were demonstrated by confocal immunoflurescence， and they were all positive. Then we used Oct4， Sox2， Klf4 and c-Myc to transfect FL-MSCs and got the human embryonic stem cells-like (hES-like) clones. We used karyotype and STR analysis to prove that these clones were derived from the FL-MSCs， and then we got the preliminary evidence about that these clones were iPS by teratoma experiment.
CN
EN