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The influence of 3-Nitrotyrosine to Oxidative Stress in HepG2 Cell
Yu Jing1, Xu Yuanyuan1, Chen Lili1, Li Zhuqing1, Le Guowei1,2, Shi Yonghui1,2*
1Institute of Food Nutrition and Functional Factor, School of Food Science and Technology, Jiangnan University,Wuxi 214122, China; 2The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Abstract: In order to determine whether 3-nitrotyrosine can promote oxidative stress in cells， HepG2 cells were treated with 3-nitrotyrosine at different time and concentrations. And the cell viability， intracellular ROS (H2O2， O2–)， total antioxidant capacity of cells， intracellular antioxidant enzymes and lipid oxidation were tested. The results showed that， with high concentration (300 μmol/L) of 3-nitrotyrosine， the cell viability decreased to 48.5% when incubated for 48 h. Meanwhile， 3-nitrotyrosine greatly affected intracellular ROS， intracellular antioxidant enzyme activity， and intracellular lipid oxidation of the cells. And it was proportional to 3-nitrotyrosine content and reaction time. In addition， 3-nitrotyrosine reduced mitochondrial membrane potential， and the expression of SirT3. In conclusion， 3-nitrotyrosine acted not only as protein oxidation products， but also could exacerbate oxidative stress of the cells by reducing the antioxidant capacity of the body and eventually lead to apoptosis.
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