GABA Promoted the Proliferation of Neural Stem Cells from the SVZ of Adult Mice

Jin Zhenchao¹, Deng Xiaolan¹, Wang Jian², Zheng Min^1*

¹Department of Stem Cells and Tissue Engineering of Chongqing Medical University, Chongqing 400016, China; ²Neurology Department of Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Abstract: The Alarmar blue， BrdU incorporation assay and fluorescence activated cell sorter were used to investigate the effect of γ-aminobutyric acid (GABA)， an important inhibitory neurotransmitter in the nervous system， on proliferation of neural stem cells (NSCs) from adult mouse subventricular zone (SVZ). The results showed that with intervention of different concentrations of GABA and BICC， the proliferation of NSCs was significantly enhanced in GABA group， and was decreased in BICC group; In GABA group， the proportion of NSCs in active proliferation period was significantly higher than blank control group， while the proportion in BICC group was significantly lower (P<0.05)， and the proportion of cells in S-G2 period was significantly increased (P<0.05) in GABA group. The results indicated that GABA can promote proliferation of NSCs from SVZ zone of adult mouse by promoting them into active proliferation period.

CSTR: 32200.14.cjcb.2014.04.0013