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Effect of T-lymphocytes on Cell Proliferation and Osteogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells
Shao Bingyi1,2, Yu Yang1,2, Fu Xiaohui3, Liao Li4, Jin Yan4, Yang Deqin1,2*
1Department of Endodontics, the Affiliated Hospital of Stomatology. The Stomatology College The Chongqing Medical University; 2Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China; 3T
Abstract: To investigate the effect of T-lymphocytes on the cell proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMMSC)， the bone marrow-derived mesenchymal stem cell was co-cultured with T-lymphocytes from the sham， OVX group， sham+anti-TNF-α and OVX+anti-TNF-α group. The MTT method was used to analyze the growth curves of BMMSC. After osteogenic induction， calcium nodules were observed by alizarin red staining and alkaline phosphatase staining. The expression levels of BMMSC osteogenesis related genes， for instance， Runx2， OCN were measured by RT-PCR. Compared with sham group， the proliferation capacity of BMMSC in OVX group was declined， and the osteogenic potential was decreased. Compared with OVX group， the proliferation capacity of BMMSC in OVX anti-TNF-α group was increased， and the osteogenic potential was enhanced. The results demonstrated that T-lymphocytes might influence the osteogenic activity of BMMSC by inhibiting proliferation and osteogenic differentiation under estrogen deficiency， which might be related to the increased expression of TNF-α by T-lymphocytes.
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