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IL-12 Overexpression Regulate the Differentiation of Monocytic Tumor Cell to Macrophagocyte
Ma Tingting, Xiong Haiyu, Wang Qin, Cheng Feng, Li Ziwei, Wu Bitao, Lin Yan, Tu Zhiguang*
College of Laboratory Medicine, Key Laboratory of Laboratory Medical Diagnostics of Education Ministry, Chongqing Medical University, Chongqing 400016, China
Abstract: In this study， whether IL-12 overexpression could regulate differentiation of monocytic tumor cell to macrophagocyte was investigated. Firstly， a monocytic tumor THP-1 cell model overexpressing IL-12 was established. Furthermore， the change of cell morphology， matured macrophagocyte marker expressions and phagocytosis were detected. Morphologically， the experimental group cells were round or irregular round shape， and some cell membrane processes were observed; cell nucleolus became fuzzy or completely disappeared; the nuclear chromatins appeared to be dense and cordlike. Compared with the control group and empty vector group， CD68 mRNA and protein expressions were up-regulated (P<0.05). However， compared with blank group， CD11b mRNA and protein expressions had no remarkable change in the IL-12 overexpression group (P>0.05)， whereas CD11b protein expression in experimental group was significantly higher than that in empty vector group (P<0.05). Moreover， phagocytosis of the experimental group was significantly better than that of the control group and empty vector group (P<0.05). In conclusion， IL-12 overexpression could promote the differentiation of monocytic tumor cell to macrophagocyte.
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