Home > Browse Issues > Vol.36 No.4
The Effect of Peripheral Nerve Tissue Fluid on the Differentiation of Rat EPI-NCSCs In Vitro
Zhang Lu1,2,3, Zhang Jieyuan1, Liu Bin2,3, Liu Zheng4, Li Bingcang1*
1Research Institute of Surgery, Daping Hospitai/State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China; 2School of Life Science, South West University, Chongqing 400715,
Abstract: In order to explore environmental factors of peripheral nerve for EPI-NCSCs differentiation， we used sciatic nerve tissue extract as inducing factors for EPI-NCSCs (epidermal neural crest stem cells) in vitro differentiation. Normal culture EPI-NCSCs were used as control in culture medium containing DMEM/F12， 10% FBS (fetal bovine serum)， 2% B27 and 20 ng/mL bFGF (basic fibroblast growth factor). Peripheral nerve tissue fluid (1%) was added to cell culture medium as the experiment group. MTT showed that peripheral nerve tissue fluid added into culture medium had no effect on the survive and proliferation of EPI-NCSCs compared with those of the control group; EPI-NCSCs showed different morphology in the control group and experiment group; RT-PCR analysis showed that GFAP， S-100 and β-III Tubulin (Tuj1) were expressed in the control group and experiment group. However， the expression levels of three proteins in the experiment group were higher than those in the control group. Immunohistochemical analysis revealed that in the control group EPI-NCSCs appeared to be β-III Tubulin positive. In the experiment group， EPI-NCSCs were S-100 and GFAP positive through immuocytochemistry. All these results demonstrated that peripheral nerve tissue fluid (1%) could be added to culture medium to induce EPI-NCSCs to differentiate towards Schwann cells in vitro.
CN
EN