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Sorting the Florescence Positive Cells in Transgenic Zebrafish Embryo by Fluorescence-activated Cell Sorting
Wang Xin1, Zhang Suzhen2, Wang Xueqian1, Li Liping1, Zhang Jingjing2*, Liu Dong1*
1Jangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China; 2Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
Abstract: The florescence proteins are used to specifically label organs， tissues or cell types in transgenic zebrafish reporter lines， which are extensively applied in developmental biology and disease modeling. They allow us to follow the several-labeled cells in vivo. In this paper， we introduced the procedure to sort the florescence positive cells in transgenic zebrafish embryo by fluorescence-activated cell sorting (FACs). Based on our experience of several experimental tests， this procedure was optimized to be very efficient and easy to handle.
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