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The Effects of DEPDC1 Depletion on Growth and Cell Cycle in Nasopharyngeal Carcinoma Cell Line HNE-1
He Longxia1, Mi Yan1, Zhang Chundong2, Zhang Ying2, Cai Wei2, Wang Yitao2, Bu Youquan2, Zhu Jiang1*
1Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the potential role of DEPDC1 in nasopharyngeal carcinoma， the specific siRNAs against DEPDC1 was designed， synthesized and transiently transfected into the nasopharyngeal carcinoma cell line HNE-1. The results of RT-PCR and Western blot clearly demonstrated that the expression of DEPDC1 was efficiently inhibited at both mRNA and protein levels. FACS (fluorescence activated cell sorter)， MTT and apoptosis assays demonstrated that DEPDC1 depletion resulted into the G2/M arrest， growth retardation and apoptosis. In addition， results of RT-PCR showed that DEPDC1 depletion caused the upregulation of A20 gene， an inhibitor of NF-κB activation， and the downregulation of several NF-κB target genes such as C-MYC， MMP9， ICAM-1 and BCL-2. Taken together， our results suggested that siRNA-mediated DEPDC1 depletion caused cell cycle arrest，led to growth inhibition induced apoptosis in the nasopharyngeal carcinoma cell line HNE-1， which were possibly mediated by the inactivation of NF-κB pathway.
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