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Mithramycin A Inhibited Expression of MRP1 Gene of Human Lung Adenocarcinoma Cell A549/DDP
Li Huaiyong, Shao Shuli*, Zhang Weiwei, Yang Xiting, Liu Rui, Chen Chuang, Che Jiaxiang, Xu Junyi
College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
Abstract: The study investigated the effects of Mithramycin A on the expression of MRP1 gene of human lung adenocarcinoma cell A549/DDP. The inhibitory effect of Mithramycin A on the growth of A549/DDP cells was tested by MTT method in vitro. The levels of Sp1/MRP1 were measured by Real time RT-PCR and Western blot. The results showed that Mithramycin A inhibited the growth of A549/DDP cells in a dose-dependent manner.After exposure to 300 nmol/L Mithramycin A for 48 h， the expressions of mRNA and protein of Sp1 and MRP1 were reduced. The results suggested that Mithramycin A depressed the expression of MRP1 gene of human lung adenocarcinoma cell A549/DDP through down-regulation of Sp1 gene.
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