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Comparative Study on the Mechanical Characteristics of the Mice Lung Cells and Lewis Lung Carcinoma
Niu Qingyuan, Zhang Jingjing, Ji Zhenling, Li Jianting, Ma Deshun*
College of Life Science and Engineering of Shenyang University, Shenyang 110044, China
Abstract: In this article， we observed and compared the microfilament differences with phalloidin staining technology， determined the α-SMA protein changes of single cell with fluorescent antibody staining technology，and researched the changes of the cell traction force with CTFM (Cell Traction Force Microscopy) method between the mice lung cells and lewis lung carcinoma. The results showed that the microfilament in lewis lung carcinoma changed， and the cell morphology was also affected. α-SMA protein was significantly decreased and dispersed; The cell projection area was significantly reduced by roughly 27%， and the cell traction force was significantly reduced (RMS was reduced by roughly 49%) in lewis lung carcinoma. These results suggest that the cell skeleton， cell morphology， α-SMA protein and cell traction force are closely related to the process of canceration.
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