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Cloning and Expression Analysis of Phytoene Desaturase Gene in Ornamental Sunflower
Wu Jianshe1,2,3, Zhong Huaiqin1,2,3, Huang Minling1,2,3*
1Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2Flowers Research Center, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 3Fujian Engineering Research Center of Ch
Abstract: The HaPDS gene cDNA sequence involved in carotenoids synthesis was cloned from the yellow petals of Ornamental Sunflower ‘MinKui 3’ using RT-PCR and RACE techniques. The cDNA sequence consists of 2 017 bp with an intact open reading frame of 1 710 bp， encoding a polypeptide of 570 amino acids. Homology analysis showed that the deduced HaPDS protein was highly homologous to other PDS proteins from different species. The putative protein sequence has a cofactor binding-domain in N-terminal， and a carotene binding-domain in C-terminal. Phylogenetic analysis indicated that HaPDS was more related to PDS of Tagetes erecta and Chrysanthemum x morifolium. Quantitative RT-PCR analysis indicated that HaPDS showed the highest transcript abundance in full bloom. HaPDS was highly expressed in ray florets， bracts and leaves， but lowly expressed in tubular florets. With the increasing in the amount of MkPDS relative expression， colour changed from white to yellow and golden transition.
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