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Screen for Membrane and Lipid Proteins Required for the Subcellular Localization of ScRch1 in Budding Yeast
Zhao Gang, Cao Chunlei, Zhao Yunying, Jiang Linghuo*
School of Biotechnology, the National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China
Abstract: Saccharomyces cerevisiae YMR034c is a sequence homolog for the Candida albicans CaRCH1 gene， named as ScRCH1， and we show that ScRch1 localizes to the plasma membrane in response to high levels of extracellular calcium. To find out membrane and lipid proteins related to the subcellular localization of ScRch1， we screened 402 yeast single-gene deletion mutants for genes encoding membrane and lipid proteins through a fluorescence microscope approach. We have identified 10 genes， whose deletion renders ScRch1-GFP failed to localize to the plasma membrane. These genes include SNQ2 and HXT1 encoding plasma transport proteins， AVT4 encoding vacuolar transport proteins， PEP3 encoding docking/fusion tonoplast protein， DFG10 related to cell differentiation，EHT1 and LDH1 encoding liposome proteins， as well as YBR219c， YBR224w and YDR417c with unknown functions.These data provide a basis for our understanding the regulatory mechanisms of ScRch1 subcellular localization.
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