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Cloning and Expression of Anthocyanidin Synthase in Strelitzia reginae Banks
Fan Ronghui1,2,3, Huang Minling1,2,3*, Wu Jianshe1,2,3, Zhong Huaiqin1,2,3
1Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou 350013, China;2Flowers Research Center , Fujian Academy of Agricultural Science, Fuzhou 350013, China;3Fujian Engineering Research Center for Char
Abstract: In the study， the full-length cDNA sequence of ANS gene (SrANS) was cloned from blue petals of Strelitzia reginae Banks using RT-PCR and RACE techniques. The cDNA sequence was 1 389 bp and included a whole open reading frame of 1 083 bp， encoding a polypeptide of 361 amino acids. The amino acid was highly conserved compared with other ANS homologues and shared up to 74% homology with ANS from Iris hollandica. Phylogenetic analysis indicated that SrANS was more related to ANS from Anthurium andraeanum. The conserved structural domain analysis revealed that SrANS had the typical functional domains of ANS protein， containing 2-oxoglutarate and iron ion combination sites and belonging to the 2OG-Fe(II)-dependent dioxygenase superfamily. The semi-quantitative PCR analysis indicated that SrANS was highly expressed in mid-bud period and early flowering period， and the transcript level was highest in blue petals.
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