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Effect of Umbilical Cord Mesenchymal Stem Cell Transplanting on Retinoicacid-induced Osteoporosis in Rats
Liu Qiuying 1 , Li Yicheng 1 , He Shaoqing 2 , Luo Zhenyu 1 , Ma Yanyan 1 , Lin Yongliang 1 , Luo Yong 1 ,Qian Chuiwen 1 , Wang Yifei 1*
1Biomedical Research and Development Centre of Jinan University, Guangzhou 510632, China;2Wanzai Middle School of Yichun in Jiangxi Province, Yichun 336100, China
Abstract: To observe the therapeutic effect of umbilical cord mesenchymal stem cell on retinoicacid induced osteoporosis in female rats by intravenous injection， forty SD female rats were divided randomly into four groups: control group (Group A)， model group (Group B)， hUC-MSCs transplantation (Group C)， and Xianling Gubao group (Group D). The rats in Group B， C and D were received retinoic acid at 70 mg/(kg·d) by gastric lavage for 14 days to induce osteoporosis instead of sterile water in Group A. At the same time， Group C were adiministrated with 2×106 hUC-MSCs， Group D adiministrated with 20 mg/kg Xianling Gubao， and Group B were administrated with saline. After 21 days， the serum biochemical indicators， biomechanics characteristics were measured and microstructure of bone were also observed. The results showed that the concentration of Ca2+， Pi and ALP was increased appararently after RA induced， and it was recovered in Group B and C. The concentration of StrACP was increased in Group B and decreased in Group D vs Group A， but there was no difference in Group C. The wet weight of right femur was extremely decreased in Group B than Group A， and there was no difference statistically between Group C and D. The BMD was decreased after RA induced in Group B than Group A， and there was no difference between Group C and D. Examination of the collagenous fibers arrangement showed osseous collagen were deranged and surface loosening. Meanwhile， there was indication of repair in Group C. Taken together， these data suggested that RA-induced osteoporosis in rats could be relieved by injecting with umbilical cord mensenchymal stem cells.
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