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Study on the Spatial Organization of Bcl11b Gene Locus in Cell Nucleus with the Circular Chromosome Conformation Capture Techniques
Ren Licheng1, Li Meiying2, Sun Yuantian1, Su Zhenyu1, Yang Zhi3, Li Dongna1*
1Department of Biology, Hainan Medical University, Haikou 571199, China; 2Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;3Department of Morpholog
Abstract: The genome-wide gene loci can be captured by the circular chromosome conformation capture (4C) technique， which potentially interacted with the target gene locus of the 4C assay. The spatial organization of the 4C target gene locus in cell nucleus can be further investigated using the 4C-related techniques. In this article， the Bcl11b locus was choosen as the target gene locus for 4C assay. Based on the serials of optimization experiments for the PCR conditions， the nested-reverse PCR was efficiently amplified for 4C assay. Combined with the limited clone screening and the general DNA sequencing techniques， many genome-wide gene loci were captured with the 4C assay， which potentially interacted with the Bcl11b gene locus. These interactions between the gene loci and the 4C target gene locus were occurred in inter-chromosome and intra-chromosome. The interactions between these gene loci showed the complex spatial organization of Bcl11b locus in cell nucleus.
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