The Protective Effect of Glutathione on Chromosome Aberration of Mice Induced by Cisplatin

Abstract: To study the effect of glutathione (GSH) on chromosome aberration induced by cisplatin (CDDP)， Kunming mice in the experiment were randomly divided into the control group， GSH group， CDDP group， and group of GSH+CDDP. The mice of GSH Group were injected with Glutathione by tail vein in accordance with 1 200 mg/(kg·d) in three days. The control group were injected with the same volume of normal saline. The CDDP group were injected once with Cisplatin of 20 mg/kg on the 2nd day. The blood sample was drawn by the tail vein of mice for routine blood test. Kill the mice and get the bone marrows for nucleated cell count， micronucleus test and chromosome G banding. Read the slides with double-blind method and carry out the statistical analysis. The results showed that， for the control group， the numbers and morphology of the chromosome were regular， and the micronucleus was seldom observed. The comparison between the GSH group and the control group showed no difference (P>0.05). For the mice of CDDP group and GSH+CDDP group， the bone marrow depression was distinctly， and the chromosome aberration rates and micronucleus rates were significantly increased， which were significantly different from the control group (P<0.01). The aberration rate in the GSH+CDDP group was significantly lower than that in the CDDP group (P<0.05). These findings suggest that the glutathione has a definite protective effect on the chromosome damage induced by cisplatin.

CSTR: 32200.14.cjcb.2013.10.0011