Effect of A2AR Knock out on Astrocyte Activation in SVZ of Focal Cerebral Ischemia-reperfusion in Mice

Abstract: This article investigates the effects of adenosine A2A receptor (A2AR) on astrocyte (Ast) activation in subventricular zone (SVZ) during focal cerebral ischemia-reperfusion in mice， to provide experimental evidences for application of adenosine drugs on Stroke. Focal cerebral ischemia-reperfusion was induced by middle cerebral artery occlusion (MCAO). The mice were randomly divided into Sham operated groups (S)， model groups (M)， and the model groups were divided into A2AR-gene-ko-model-groups (MKO) and A2AR-gene-wt-modelgroups (MWT). According to the time after ischemia-reperfusion， MKO groups and MWT groups were divided into 1 d groups， 3 d groups and 7 d groups. And the S groups were divided into A2AR-gene-ko-sham-groups (SKO) and A2AR-gene-wt-sham-groups (SWT). Neurological behavior was assessed on each mice. Brain slices were observed for infarction by 2，3，5-triphenyltetrazolium chloride (TTC); general shape of brain was observed by HE&Nissl staining; and the specific markers glial fibrillary acidic protein (GFAP) of Ast was measured by Immunofluorescence.Abnormal neurological behavior was observed in the animals of M groups， but the neurological behavior of mice in MKO 3 d group was better than these in the MKO 1 d group (P<0.05). Typical cortical infarct lesion in M groups was found by TTC staining. The expression of GFAP in the brain of S groups was rather little， which was enhanced significantly in M groups; besides， the expression of GFAP in the MKO 7 d group was less than that in the MWT 7 d group. A2AR-gene-ko can mitigate handicap during acute stage of ischemia reperfusion， regulate activation of astrocyte during convalescence， which should be one of favorable factors for neuranagenesis.

CSTR: 32200.14.cjcb.2013.08.0006