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mRNA Profiles for Phase II Metabolizing Enzymes in Livers of Male and Female Mice
Liu Ying1, Liu Nanqi1, Jiang Kunyu2, Meng Shengnan2*
1Department of Biochemistry, College of Basic Medical Science, China Medical University, Shenyang 110001, China; 2Department of Pharmaceutics, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China
Abstract: Gender differences in mRNA profiles for phase II metabolizing enzymes were characterized in livers of male and female mice of 6 months of age. C57BL/6 mice were randomly divided into female group and male group (n=6)， the livers were collected. The relative mRNA expression of phase II metabolizing enzymes was examined by Real-time quantitative PCR (RT-qPCR). Gender differences were investigated for 19 phase II metabolizing enzymes. In female and male mice， mRNA levels for 4 phase II metabolizing enzymes (e.g.， Ugt1a1， Sult1a1， Sult2a2 and Sult3a1) were higher in female mice (P<0.01 or P<0.05)， whereas mRNA levels for 6 phase II metabolizing enzymes (e.g.， Ugt2b1， Ugt2b5， Ugt2b35， Sult5a1， Gstp1 and Gstp2) were higher in male mice (P<0.01 or P<0.05). The mRNA levels of the other enzymes showed similar levels in both genders. Gender differences have significant effects on the mRNA expression profiles of phase II metabolizing enzymes in livers of mice， and it may contribute to individual differences in drug efficacy and toxicity.
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