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O-GlcNAcylation of Functional Protein Regulates Human Choriocarcinoma Cells Migration
Qu Runan, Ju Nana, Wu Mingjun, Zhao Dezhang, Wang Yingxiong, Yang Zhu, Yu Chao*
Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the molecular mechanisms involved in human choriocarcinoma cells (JAR) migration regulated by O-GlcNAc glycosylation， cell models expressing different O-GlcNAcylation levels were established by siRNA interference or OGA inhibitor. Transwell assay and adhesion assay were used to examine JAR cell migration ability and adhesion to EA.hy926 cells. Meanwhile， the O-GlcNAcylation level of β-catenin was detected by immunoprecipitation. The results indicated O-GlcNAcylation enhances the JAR cell migration and adhesion to endothelial cells in vitro. Moreover， the O-GlcNAcylation of β-catenin but not the protenin level increased apparently. The migration and adhesion ability were obviously declined in JAR cells with O-GlcNAcylation down-regulation. These findings suggest that O-GlcNAcylation is involved in the regulation of tumor cell migration.
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