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Establishment of Visible Microencapsulated Cell Co-culture Separation System
Wu Hao1,2, Ma Ying1,2, Shan Shuang1, Tan Mingqian1*, Ma Xiaojun1*
1Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023,China; 2University of Chinese Academy of Sciences, Beijing 100049, China
Abstract: Cell microencapsulation co-culture has been proved to be a valuable technology in the fields of cell induced differentiation， cell proliferation regulation and improving the production of metabolites. Visible identification and separation of two kinds of microencapsulated cells play an important role in cell co-culture. Fe3O4 nanoparticles (Fe3O4 NPs) as a kind of paramagnetic material can be used for microencapsulated cell separation. In this study， microencapsulated cell co-culture system， fluorescent visible paramagnetic microcapsules were developed by doping water soluble Fe3O4 NPs into alginic acid-fluorescent chitosan microcapsules (AC microcapsules) membrane for co-culture of Chinese hamster ovary cell (CHO). The results showed that two kinds of cell culture microcapsules could be identified by fluorescence signal and separated by magnetic driving in the process of cell co-culture. The fluorescent visible paramagnetic microcapsules system shows a great potential for cell co-culture.
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