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Effect on the Expression of Macrophages Transferrin Receptor and Ferritin in Macrophages by Mycobacterium Tuberculosis
Li Wei, Dong Weijie, Liu Yunxia, Tuo Qingzhang, Liu Danxia, Wu Fang, Zhang Le,Wu Jiangdong, Zhang Wanjiang*
Department of Pathophysiology, Medical School, Shihezi University/Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University, Shihezi 832002, China
Abstract: To research the changes of expression levels of transferrin receptor and ferritin， and analyze its significance in different phases， macrophages infected with virulent Mycobacterium tuberculosis strain H37Rv (hereinafter referred to as H37Rv strains) and BCG vaccine strain (hereinafter referred to as the BCG strain)， respectively， the expression of TfR and Fn in the supernatants was detected at 1， 6， 12， 18， 24 h after infection with ELISA and Western blot technologies. ELISA technologies demonstrated that the TFR expression in the infected group was higher than that in the control group， the results showed that the expression reached the peak 12 h and 18 h after infection， which showed the most obvious differences (P<0.05). The results of Western blot indicated that the differences between the expression levels detected 1， 6， 18 h after the models created were statistically significant (P<0.05). Detected by ELISA and Western blot， the expression of Fn in groups of mice macrophages increased gradually over the time. The result showed Fn expression in the control group was higher than that in the infected group at 1 h. After 6 h infection， Fn expression in the infected group wass higher than that in the control group; the H37Rv group>the control group at 12 h and there was statistical significance (P<0.05); the H37Rv group>BCG group>control group at 18 h and 24 h and there was statistical significance (P<0.05). Infection of Mycobacterium tuberculosis induced the rise of expression of TfR and Fn in macrophages.
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