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In vivo Dynamic Ca2+ and K+ Oscillation Responding to GABA Reveals Ion Channels Participating in Tobacco Pollen Tube Growth Regulation
Zhao Li1, Zhao Zhilong2, Gong Hanyu1, Qin Yonghua1, Yu Guanghui1*
1College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China; 2Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
Abstract: γ-aminobutyric acid (GABA) plays an important role in pollen tube growth， however， the molecular mechanism underlying is far from being clarified. Here， we present evidences to show that GABA could regulate the flux direction of Ca2+， K+ using Non-invasive Micro-test Technique. The results indicate that GABA could specially facilitate Ca2+ influx and trigger K+ outflux across the membrane on the tip of tobacco pollen tubes.The experiment applying specific inhibitor of permeable Ca2+ channel shows that this Ca2+ currency is specially induced by the activation of permeable Ca2+ channel. This Ca2+ oscillation pattern is very different from the pattern caused by Glutamate. Our results imply that GABA may regulate Ca2+ channels and K+ channels via the activation of putative GABA-B type receptor on tobacco pollen tube cell membrane， and lead to Ca2+ influx and K+ outflux to modulate tobacco pollen tube polar growth and morphogenesis.
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