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Expression of Lipogenic Genes in Diannan Small-ear Pigs with Different H-FABP Genotypes
Li Zhijuan, Gao Shizheng, Pan Hongbin, Huang Ying, Yang Minghua, Zhao Sumei*
Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, China
Abstract: The intramuscular fat (IMF) is the main factor affecting meat quality traits. In fact， intramuscular fat is triglycerol (TG) depositing in intramuscular adipocytes. The contents of TG were decided by the lipid metabolism. This study analyzed the expression levels of lipid synthesis metabolism related genes in Diannan smallear pigs with different H-FABP genotypes using Real-time PCR， and measured the correlation between the content of TG and the expression levels of genes. The results showed that Diannan small-ear pigs had no polymorphism at HaeIII-RFLP， MspI-RFLP location， and displayed genotypes of DD， AA， respectively. Diannan small-ear pigs had polymorphism at HinfI-RFLP location， and displayed genotypes of HH， Hh， hh. The contents of TG in Diannan small-ear pigs with different H-FABP genotypes were HH>Hh>hh. The mRNA expression levels of lipogenic genes， including H-FABP， A-FABP， SCD， ACC， FAS and DGAT-1 were significantly higher in HinfI PCR-RFLP HH genotypes than in other genotypes (P<0.05). The mRNA expression levels of H-FABP， A-FABP， SCD， FAS， ACC and DGAT-1 genes and the content of TG in intramuscular adipocytes had significant positive correlation (P<0.01).In conclusion， the H-FABP genotypes affected the intramuscular fat content in Diannan small-ear pigs. The Mrna expression levels of lipogenic genes in intramuscular adipocytes and the content of TG in intramuscular adipocytes had significantly positive correlation.
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